Wiper Party leader Kalonzo Musyoka has bolted out of Azimio la Umoja - One Kenya coalition.
Kalonzo said One Kenya Alliance will now stand as it originally was.
The Wiper leader announced the move alongside KANU's Gideon Moi on Monday morning in Karen.
“We have agreed to go separate ways with Azimio La Umoja and compare notes as we do so. I wish Raila Odinga's running mate Martha Karua well,” Kalonzo said.
Makueni MP Dan Maanzo on Sunday said the party leadership met on Saturday and agreed to withdraw from Azimio la Umoja-One Kenya Coalition after talks to have Kalonzo deputise Raila Odinga failed.
"We have been negotiating for days now but Raila Odinga appears to be rigid and we have no options other than part ways," Maanzo said.
Kalonzo has announced he will vie for the presidency in the August polls and has appointed former Cabinet Minister Julius Sunkuli as his running mate.
Raila on the other hand has unveiled Martha Karua as his running mate at an event held at KICC.